Mechanisms for Defining Supercoiling Set Point of DNA Gyrase Orthologs
نویسندگان
چکیده
منابع مشابه
Gyrase inhibitors and intracellular DNA supercoiling.
cells are known to require ATP for all catalytic activities. However, during the purification of the enzymes, some of their native properties may be lost due to proteolytic degradation or to a resolution of their supramolecular structure (Glikin et al., 1984). The trypanosomal extracts, as well as the enzyme obtained after hydroxylapatite chromatography, are capable of catenating supercoiled ci...
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Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a...
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Reverse gyrase is a unique type IA topoisomerase that can introduce positive supercoils into DNA. We have investigated some of the biochemical properties of Archaeoglobus fulgidus reverse gyrase. It can mediate three distinct supercoiling reactions depending on the adenine nucleotide cofactor that is present in the reaction. Besides the ATP-driven positive supercoiling reaction, the enzyme can ...
متن کاملGyrase inhibitors can increase gyrA expression and DNA supercoiling.
Treatment of bacterial cells with inhibitors of gyrase at high concentration leads to relaxation of DNA supercoils, presumably through interference with the supercoiling activity of gyrase. Under certain conditions, however, the inhibitors can also increase supercoiling. In the case of coumermycin A1, this increase occurs at low drug concentrations. Oxolinic acid increases supercoiling in a par...
متن کاملMicrococcus luteus DNA gyrase: active components and a model for its supercoiling of DNA.
Two active components alpha and beta of micrococcus luteus DNA gyrase, of peptide weights of 115,000 and 97,000, respectively, have been purified. Each individual component exhibits little DNA gyrase activity; the ATP-dependent negative supercoiling of a covalently closed circular DNA duplex is catalyzed by a combination of the two. Covalent closure by Escherichia coli ligase of a circular DNA ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2012
ISSN: 0021-9258
DOI: 10.1074/jbc.m112.345678